Investigating expression of SOX12 and its inhibition effect on induction of apoptosis in human Acute Myeloid Leukemia cell line (KG-1) by Antisense GapmeRs.

سال انتشار: 1399
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 338

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شناسه ملی سند علمی:

ICIBS01_083

تاریخ نمایه سازی: 2 آذر 1399

چکیده مقاله:

Introduction & Objectives: Acute myeloid leukemia (AML) is a heterogeneous hematopoietic malignancy characterized by the rapid accumulation and malignant proliferation of immature myeloid progenitors. It is the most common leukemia in adults with a high incidence and mortality rate especially in elderly. SOX12 is a novel potential target for acute myeloid leukemia however; the role of SOX12 in leukemia remains unknown.Materials & Methods: In this study, SOX12 was blocked by antisense GapmeRs in human Acute Myeloid Leukemia cell line (KG-1). Cells were transected with SOX12 antisense GapmeRs at 24, 48, and 72 h post-transfection. Quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) was accomplished to evaluate the SOX12, Twist1, CASP3 and CASP9 expression.Results: Our results showed that SOX12 inhibition can reduce expression of Twist1. In addition, downregulation of SOX12 in KG-1 cells could induce apoptosis, probably through the upregulation of CASP3 and CASP9.Conclusion: The findings declare that inhibition of SOX12 could be a new target in the treatment of AML and approach treatment based on antisense therapy.

نویسندگان

Arezou Dabiri

Department of Immunology, Shahid Sadoughi University of Medical Sciences, Yazd, Iran

Mohammad Reza Sharifi

Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan ۸۱۷۴۴-۱۷۶, Iran

Akram Sarmadi

Genetics and Molecular Biology Dept. Isfahan University of Medical Sciences. Isfahan. I.R. Iran