The study of the protective effect of vitamin E and Retinoic acid on testicular tissue in mice treated with cyclophosphamide

Introduction: One of the side effects of anticancer drugs is spermatogenesis disorder and the evaluation of sperm production after chemotherapy has been the subject of several studies today.materials and methods: Adult male mice weighing 20 5 5 g were randomly divided into 5 groups (n = 4) Control, cyclophosphamide (12 mg / kg / day), cyclophosphamide and vitamin E (200mg / kg / day), cyclophosphamide and retinoic acid (500 uL / per72h), cyclophosphamide and retinoic acid (250 ul / per72h) and vitamin E 100mg / day ) Were divided. The treatment was continued for 35 days and at the end of the rats were anesthetized and testicular tissue was isolated, weighed, fixed and stained with hematoxylin-eosin. Then, histological studies were performed and data were analyzed by SPSS software and the differences were considered significant (P≤0 / 001).Results: Significant decrease (P≤0 / 001) in the mean diameter of seminal vesicles and epithelial thickness and index of tubular differentiation was observed in the testis tissue of mice treated with cyclophosphamide compared to the control group and in each group. Cyclophosphamide recipient with retinoic acid or vitamin E parameters significantly increased to the level of control group (P≤0 / 001), whereas cyclophosphamide co-administration with retinoic acid and vitamin A had less tissue protective effect.Discussion: Vitamin E and retinoic acid each can offset the adverse effects of cyclophosphamide on spermatogenesis and may be inducers of spermatogenesis, while the combination of these two antioxidants has less efficacy.

کلیدواژه ها:

Cyclophosphamide ، Vitamin E ، Retinoic acid ، Spermatogenesis