Effect of oral B complex vitamin on sperm parameters and chromatin integrity in animal models of varicocele

سال انتشار: 1399
محل انتشار: کنگره بین المللی علوم زیست پزشکی اصفهان
کد COI اختصاصی: ICIBS01_050
زبان مقاله: انگلیسی
تعداد مشاهده: 416

نویسندگان

Majesty of Maryam Infertility Center, Martyr Beheshti Hospital, Isfahan University of Medical Sciences, Isfahan, Iran

Majesty of Maryam Infertility Center, Martyr Beheshti Hospital, Isfahan University of Medical Sciences, Isfahan, Iran- Anatomical Sciences Research Center, Kashan University of Medical Sciences, Kashan, Iran- Gametogenesis Research Center, Kashan Universi

Mahnaz Fadaee

Majesty of Maryam Infertility Center, Martyr Beheshti Hospital, Isfahan University of Medical Sciences, Isfahan, Iran

چکیده

Objectives: Varicocele is abnormal dilation and torsion in the pampiniform plexus veins of the spermatic cord. In the past decade some clinical tests approved positive effects of oral antioxidant on oxidative stress in sperm. Vitamin B is indispensable for DNA replication and synthesis, and also metabolism of amino acid and fatty acid. It acts as a coenzyme for methionine synthase enzyme in one carbone cycle that synthesises methionine from homocysteine and results in the methylation of the main functional macromolecules/molecules such as DNA, RNA, lipids, proteins, and amino acids. Homocysteine directly association with increased oxidative stress. Many studies have shown that varicocele associated with increased levels of oxidative stress on sperm and deacrease semen antioxidant capacity. Materials and methods: Male rats (n = 40) were divided into control (n = 10), sham (n = 10) and varicocele induction (n = 20) groups. From the 20 remaining rats in varicocele group, 10 rats received Vit B complex (6, 9.6, 30.4, 9.6 and 0.006 mg/kg for B6, B2, B9, B1 and B12 respectively), 10 rats only received water for 2 months. all animals were killed and both testicles were removed, and sperm were collected from the left epididymis tail. sperm parameters, DNA damage, lipid peroxidation and residual histone were adversely effected in the all groups.Result: we demonstrated that sperm parameters such as concentration, percentage of sperm motility and abnormal morphology was significantly lower in left testis of antioxidant supplements treated with B vitamin group compared to the other groups (p< 0.05). While, percentage of DNA damage, histone replacement and lipid peroxidation level was significantly decreased in left testis of treatment group compared to other groups (p< 0.05).Conclusion: These results showed that Vit B antioxidant was more efficient in improvement of sperm parameters, chromatin integrity and lipid peroxidation in varicocelized rats compared with others groups.

کلیدواژه ها

Varicocele, sperm chromatin, oral B complex vitamin, sperm parameters, lipid peroxidation

مقالات مرتبط جدید

اطلاعات بیشتر در مورد COI

COI مخفف عبارت CIVILICA Object Identifier به معنی شناسه سیویلیکا برای اسناد است. COI کدی است که مطابق محل انتشار، به مقالات کنفرانسها و ژورنالهای داخل کشور به هنگام نمایه سازی بر روی پایگاه استنادی سیویلیکا اختصاص می یابد.

کد COI به مفهوم کد ملی اسناد نمایه شده در سیویلیکا است و کدی یکتا و ثابت است و به همین دلیل همواره قابلیت استناد و پیگیری دارد.