L- Carnitine and fibrin encapsulation effects on restoration of estrous cycle and ovarian function after autotransplantation in mice

Acceleration of revival of ovarian function and maintaining of follicular reserve is necessary after grafting of ovarian tissue. The objective of this study was to evaluate the L-Carnitine (LC) effect and fibrin scaffold on restoration of estrous cycle and serum follicle stimulating hormone (FSH). NMRI mice were divided into six groups: Control (non- grafted), T (autografted), LC (autografted + LC), F (autografted + fibrin), LC+ F (autografted + LC + fibrin), Saline (autografted + saline). 6-weeks-old mice were bilaterally ovariectomized in the diestrus stage; then, left ovarian tissue were encapsulated in fibrin and transferred into back muscle. LC (200 mg/kg) was injected intraperitoneally one day before surgical operation and repeated until one week after grafting. Daily vaginal monitoring was performed until re-initiation of first full estrous cycle. Thereafter, follicular preservation, serum follicle stimulating hormone (FSH) levels and apoptosis incidence were assessed. Restoration of first full estrous cycle and apoptotic index in all transplanted groups were approximately similar and. however, the total numbers of follicles in transplanted groups were lower than control group. Moreover, the level of FSH in all transplanted groups was reduced significantly compared to the ovariectomized mice. In conclusion, our study, demonstrate that LC and fibrin encapsulation did not show any negative effect on transplants, and it seems that usage of scaffold in combination with a growth factor could improve the grafting results and further studies are needed in this area.