غلامرضا حبیبی
اسمعیل نیا
بزرگی
محمدی پور
مروتی
بردبار
نصیری
غلامرضا کریمی

Leishmaniasis is one of the most important protozoan parasitic diseases with different clinical manifestations. Cutaneous leishmaniasis is the common form of disease in Iran with significant consequences in terms of health. The in vitro culture of leishmania is important for diagnosis and biology study, immunology and preparation of candidate immunogens for evaluation as experimental vaccines since the past decades to now. The procedure of the leishmania culture was based on using a basic medium and enrichment materials. The fetal calf (bovine) serum is the most important component of additives for growing leishmania in in vitro. The fetal bovine serum (FBS) is very expensive and important ingredient in cell culture media. The preparation and production of FBS is very difficult. Therefore, there are too many documents show the researchers were attempted to find a substitute for FBS. In this study we focused to decrease the FBS level in media and replace with other substitutes in order to grow the leishmania major promastigotes. The results of culture of leishmania promastigotes in our proposed media with different ingredients, special concentrations and various combinations showed us the possibility of media preparation with some additives as serum substitutes for supporting the leishmania in vitro culture. The results of using our proposed media in growing the BA cell line in department of Quality Control demonstrated the potential of the media for growing the cells with high quality and quantity in comparison with the control groups. Moreover the measure of osmolality determined the media has a concentration in the range of isotonic cell culture media. The comparison of changes in the proposed medium also indicate the similar growth rate, but the intensity of cell culture was higher in proposed medium than the control positive RPMI-1640 with 10% FBS.

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