Marker-Analysis of Leaf Resistance Bacterial in Rice

  • سال انتشار: 1397
  • محل انتشار: مجله Medbiotech، دوره: 2، شماره: 3
  • کد COI اختصاصی: JR_MBT-2-3_001
  • زبان مقاله: انگلیسی
  • تعداد مشاهده: 244
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نویسندگان

Gundhu Jamal

Department of Biology, University of Dakar, Bangladesh

Sharif Rahman

Department of Biology, University of Dakar, Bangladesh

چکیده

This study was carried out with the aim of cultivating different types of fine-grain and high-growing rice. Moreover, due to the transfer of bacterial leaf blight (BLB) resistant genes, such as xa13, viz., and Xa21 from B95-1, they take advantage of Broad-spectrum resistance and durable resistance. MTU1010 (Cottondora Sannalu), as a commonly used fine-grain and high-growing rice, was chosen as the parent in the crossing procedure. In addition, this particular variety is quite vulnerable to BLB and B95-1 bearing resistant genes for BLB (Xa21 & xa13 genes). Using gene-associated primers viz., xa13 promotor and pTA 248, an experiment was carried out which proved that the target genes were widely present in B95-1. Furthermore, in order to study polymorphism between rather vulnerable (MTU1010) and resistant (B95-1) parents, the aforementioned primers were utilized as well. During Rabi, 2010 and Kharif, 2011, the cross viz., MTU1010 x B95-1 was heavily influenced and F1 progenies were firmly established. While the F1 plants were considered as true hybrids for both of the genes which progressed to the F2 generation, gene-associated markers were implemented in order to carry out the foreground selection. The genes (xa13 & Xa21) that essentially control BLB resistance obeyed Mendelian inheritance, which was suggested in a Genetic analysis of F2 populations. Finally, the plants bearing a combination of two resistance gene (xa13xa13 Xa21Xa21, xa13xa13Xa21xa21) demonstrated BLB resistance (0-2 scale), while the combinations of genes including viz., Xa13Xa13Xa21Xa21, Xa13Xa13Xa21xa21, and Xa13xa13Xa21Xa21, Xa13xa13Xa21xa21 illustrated BLB resistance (0.5-3.0), which was proposed using the phenotypic data analysis.

کلیدواژه ها

Rice, F2 population, Bacterial Leaf Blight, Marker-assisted Selection

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