Biological and physiological characteristics of human cumulus cells in adherent culture condition

  • سال انتشار: 1398
  • محل انتشار: International Journal of Reproductive BioMedicine، دوره: 17، شماره: 12
  • کد COI اختصاصی: JR_IJRM-17-12_010
  • زبان مقاله: انگلیسی
  • تعداد مشاهده: 657
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نویسندگان

Somayyeh Sadat Tahajjodi

Ph.D, Stem Cell Biology Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran- Research and Clinical Center for Infertility, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Me

Ehsan Farashahi Yazd

Ph.D,Stem Cell Biology Research Center, Yazd Reproductive Sciences Institute, Shahid SadoughiUniversity of Medical Sciences, Yazd, Iran- Department of Reproductive Biology, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran

Azam Agha-Rahimi

Ph.D, Research and Clinical Center for Infertility, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran- Department of Reproductive Biology, School of Medicine, Shahid Sadoughi University of Medical Sciences, Y

Reza Aflatoonian

Ph.D, Department of Endocrinology and Female Infertility, Reproductive Biomedicine Research Centre, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran

Mohammad Ali Khalili

Ph.D, Research and Clinical Center for Infertility, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran- Department of Reproductive Biology, School of Medicine, Shahid Sadoughi University of Medical Sciences, Y

Mahnaz Mohammadi

B.Sc, Research and Clinical Center for Infertility, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran

چکیده

Background: Cumulus cells, as oocyte nurse cells, provide a suitablemicroenvironment with growth factors and cellular interactions required for oocytematuration. Thus, these cells may serve as a natural niche for in vitro studies of femalegerm cell development. Cumulus cells may help attain a better understanding of thecauses of infertility in women and eventually improve the outcomes of cases thatrespond poorly to standard infertility treatment.Objective: The aim of this study was to isolate, culture, and investigate the biologicalcharacteristics of human cumulus cells.Materials and Methods: In this experimental study, cumulus cells were isolated,cultured, and characterized using reverse transcription-polymerase chain reactionanalyses of specific genes including FOXL2, CYP19A1, FSHR, AMHR, and LHR. Thepresence of vimentin, a structural protein, was examined via immunofluorescentstaining. Moreover, levels of anti-mullerian hormone (AMH) and progesterone secretionby cumulus cells were measured with ELISA after 2, 4, 12, 24, and 48 hr of culture.Results: In adherent culture, human cumulus cells expressed specific genes andmarkers as well as secreted AMH and progesterone into the medium.Conclusion: Cumulus cells secrete AMH and progesterone in an adherent cultureand might be applicable for in vitro maturation (IVM) and in vitro gametogenesis (IVG)studies.

کلیدواژه ها

Cumulus cells, Conditioned medium, In vitro maturation, In vitro gametogenesis, Niche

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