Evaluation of tissue-engineered chitosan-poly(vinyl alcohol)- CNT nanofibrous scaffolds in healing of rat model burn wounds

Background and Aim : Tissue engineering has provided us a new and renewable treatment which is developing very quickly and contributing wound healing process. Natural-synthetic nanofibres have recently attracted more interest because of support the cell-cell interactions and create space similar ECM. The aim of this study, investigation on the effect of chitosan-poly(vinyl alcohol)- CNT nanofibrous scaffolds in healing of rat model burn wounds. Methods : Chitosan-poly(vinyl alcohol)-carbon nanotube (Cs:PVA:CNT) nanofibrous scaffolds were electrospun from 1:2.8:1 (wt/wt) Cs:PVA solution dissolved in 80% acetic acid. In vivo study was carried out on the dorsum skin of rat which under a 2-centimeter third degree burn induction with hot water. Animals divided in three groups randomly, First group was the control group without any treatment (n=6), The medicine used for the second group was SSD ointment (n=6), and Third group was treated with Cs:PVA:CNT scaffolds(n=6). The Pathological evaluation of animals was carried out by sampling on 1st, 7th, 14th, and 21st days of treatment. Results : Macroscopic measurements of wound area showed good aspect healing effect of scaffolds in comparison with control wounds specially in 14 days post operating. Pathological studies showed that the healing rates of the wounds covered with Cs:PVA:CNT nanofibrous scaffolds were much rapid compared to untreated wounds in control group. Conclusion : The healing process of the wound covered with Cs:PVA:CNT nanofibrous scaffolds was much rapid compared to untreated wounds. However, the presence of stem cells on this scaffolds accelerated the wound healing process owing to their ability of collagen regeneration.

کلیدواژه ها:

Tissue engineering ، PVA ، chitosan ، CNT ، scaffold ، burn ، wound healing.