In vitro effect of low-level laser therapy on the proliferation and migration of human dermal fibroblasts

Background and Aim : The improvement and repair of wounds in humans with a completely complicated mechanism, and the advanced is done. It is possible that, some procedures, such as low-level laser therapy (LLLT), could accelerating the fibroblast cell proliferation and improving the wound healing process. The aim of this study was to investigate the effect of different low-level laser on proliferation and migration of human dermal fibroblast cell in vitro. Methods : Fibroblast cells were cultured in standardized conditions, stored in an oven at 37 °C, with 5% CO2 and divided into treatment and control groups. In the treatment group, fibroblast cells were irradiated with different LLLT (405,520, and 808 nm) doses every 24 h for 3 consecutive days. The rate of cellular mortality, proliferation, and migration was compared among treatments at 24, 48 and 72 hours after radiation. Results : Cellular proliferation was improved statistically significant when fibroblasts treated with LLLT (p < 0.001). Also, LLLT trigger increase of fibroblast cells migration in irradiated groups in comparison with the control group. The best dose for treatment was 808nm with 0.18 J (p < 0.001). Conclusion : These results suggest that LLLT in fibroblast cells presented an important effect on increasing cell proliferation and cell migration.

کلیدواژه ها:

low-level laser therapy ، proliferation ، migration ، human dermal fibroblasts ، In vitro