The effect of interval aerobic training and resveratrol supplementation on serum irisin, visfatin and insulin resistance in diabetic rats

It is known that irisin plays a role in regulating energy balance and body weight. Visfatin is an adipokine secreted from visceral adipose tissue and involved in glucose homeostasis. Exercise training plays a protective role against diabetes. Resveratrol is a powerful biological antioxidant. However, the role of exercise training and resveratrol supplementation on resistance insulin are not well understood. The aim of this study was to investigate the effect of moderate-intensity interval training and resveratrol supplementation on serum irisin, visfatin and insulin resistance in type ۲ diabetic rats.In this experimental study, ۴۲ Wistar rats (age: ۱۲ weeks, weight: ۲۳۰±۸ grams) were randomly assigned to ۶ groups: healthy-control, diabetic-control, diabetic+ training, diabetic+supplement, diabetic+ training+Ssupplement and saline. diabetes was induced by intraperitoneal injection of Streptozotocin (۵۰ mg/kg body weight). The training program was performed for ۸ weeks and ۳ sessions per week. Each session included ۱۰ bouts of one minute running at ۷۰% maximum speed and two minute rest between bouts. Resveratrol supplement was injected intraperitoneally daily at a dose of ۲۰ mg/kg body weight. Blood sampling was measured ۲۴ hours after the last exercise session. The enzyme-linked immunosorbent assay (ELISA) method for measuring serum irisin and visfatin levels. The data was analyzed using one-way ANOVA and Tukey post hoc tests.The results showed that irisin levels increased significantly in training, supplementation and supplementation+training groups than diabet group (P=۰.۰۰۱). Visfatin (P=۰.۰۲۱), Glucose and insulin-resistance significantly decreased in training and supplementation+ training groups than control group (P=۰.۰۱۱, P=۰.۰۰۸ respectively).Based on the findings, diabetes is associated with increased visfatin and decreased irisin and is correlated with insulin resistance. It seems that combination of moderate intensity interval training and resveratrol can have a positive effect on diabetes control by increasing irisin and decreasing visfatin, glucose and insulin- resistance levels.

کلیدواژه ها:

Diabetes ، Interval training ، Antioxidant ، Insulin resistance