The effect of growth regulators (NAA and 2,4-D) on callus induction in citrus rootstock

The present investigation was undertaken to study the effect of different concentrations and combinations of 2, 4-dichloro-phenoxyacetic acid (2, 4-D), naphthalene acetic acid (NAA) on callus induction of five citrus rootstocks (Sour orange, Citromelo, Citrange, Poncirus, Volkameriana) on MS basal medium. Different concentration of 2,4-D and NAA were tasted in order to obtain the best callus formation. Maximum callus induction response (100%) was obtained on MS medium with 2,4-D (2 and 3mg/l) and NAA (1.5 mg/l) for Volkameriana. Best callus induction response of Citrange (83.33%) was observed on MS medium with 2,4-D (1 mg/l) and NAA (1.5 mg/l). The highest frequency of the callus induction rate for Poncirus and Citromelo (91.67%) was occurred on MS medium supplemented with 2.4-D (3 mg/l) and NAA (1.5 mg/l). MS medium supplemented with 2,4-D (1 mg/l) and NAA (1.5 mg/l) showed maximum callus induction (91.76%) for Sour orange.

کلیدواژه ها:

Callus induction ، Citrus rootstocks ، 2 ، 4-D ، NAA